PDK4-IN-1 is primarily utilized in research settings to study the role of pyruvate dehydrogenase kinase 4 (PDK4) in metabolic pathways. It serves as a potent inhibitor of PDK4, which is a key regulator of glucose metabolism. By inhibiting PDK4, this compound helps researchers investigate its impact on cellular energy production, particularly in conditions like diabetes, obesity, and cancer. It is also used to explore potential therapeutic strategies targeting metabolic disorders, as PDK4 inhibition can shift cells from glucose to fatty acid oxidation, influencing metabolic flexibility. Additionally, PDK4-IN-1 aids in understanding the relationship between PDK4 and oxidative stress, providing insights into its role in various diseases.