PCI-33380 is primarily used in research settings to study its effects on specific biological pathways, particularly those involving kinases. It is often employed in biochemical assays to investigate its potential as an inhibitor for targeted therapies, especially in the context of cancer research. Scientists utilize this compound to explore its impact on cell signaling and proliferation, aiming to understand its role in modulating disease progression. Additionally, PCI-33380 is used in preclinical studies to evaluate its efficacy and safety profile, which could lead to the development of novel therapeutic agents. Its application is crucial in advancing knowledge in molecular biology and pharmacology, particularly in the design of selective kinase inhibitors.