NG25 is primarily used in research settings as a potent inhibitor of TAK1 (Transforming Growth Factor Beta-Activated Kinase 1), which plays a critical role in various signaling pathways, including those involved in inflammation and cancer. By inhibiting TAK1, NG25 helps scientists study the mechanisms of these pathways and their impact on cellular processes. This compound is particularly valuable in understanding the role of TAK1 in diseases such as rheumatoid arthritis, fibrosis, and certain cancers, providing insights that could lead to the development of targeted therapies. Its application is largely confined to laboratory studies, where it serves as a tool to dissect complex signaling networks and evaluate potential therapeutic strategies.