Necrosulfonamide is primarily used in research settings to study cell death mechanisms, particularly necroptosis. It functions as an inhibitor of mixed lineage kinase domain-like protein (MLKL), a key component in the necroptosis pathway. By blocking MLKL, necrosulfonamide helps researchers understand the role of necroptosis in various diseases, such as inflammatory conditions, neurodegenerative disorders, and cancer. Its application is crucial in identifying potential therapeutic targets for conditions where programmed cell death is dysregulated. Additionally, it aids in distinguishing necroptosis from other cell death pathways like apoptosis, providing valuable insights into cellular biology and disease pathology.