MRT67307 HCl is primarily utilized in biochemical research as a potent and selective inhibitor of TBK1 and IKKε kinases. It is often employed in studies focusing on the role of these kinases in immune responses and inflammation. Researchers use it to investigate signaling pathways, particularly those involving the regulation of interferon production and NF-κB activation. Additionally, it aids in understanding the mechanisms underlying autoimmune diseases, viral infections, and cancer, where TBK1 and IKKε play critical roles. Its application extends to exploring potential therapeutic targets for conditions associated with dysregulated kinase activity.