NQDI-1 is primarily utilized in biochemical research to inhibit the activity of apoptosis signal-regulating kinase 1 (ASK1). This inhibition is crucial for studying cellular stress responses and pathways related to apoptosis, inflammation, and differentiation. Researchers apply NQDI-1 to explore its protective effects in models of neurodegenerative diseases, where ASK1 plays a significant role in neuronal death. Additionally, it is used in cardiovascular studies to understand its potential in mitigating heart damage caused by oxidative stress. The compound's ability to block ASK1 also makes it a valuable tool in cancer research, particularly in investigating mechanisms that could lead to the development of new therapeutic strategies targeting ASK1-dependent pathways.