LDN-193189 2HCl is primarily used in research settings to study its inhibitory effects on the BMP (bone morphogenetic protein) signaling pathway. This compound is particularly valuable in stem cell research, where it helps to maintain pluripotency in stem cells by blocking BMP-induced differentiation. It is also utilized in studies focused on bone formation and regeneration, as BMP signaling plays a critical role in these processes. Additionally, LDN-193189 2HCl has been investigated in cancer research to explore its potential in inhibiting tumor growth, as BMP signaling can contribute to cancer progression. Its application extends to cardiovascular research, where it is used to study its effects on heart development and disease. Overall, this compound serves as a crucial tool in understanding cellular signaling pathways and their implications in various biological processes.