HS-1371 is primarily utilized in biochemical research as a potent and selective inhibitor of TANK-binding kinase 1 (TBK1). This compound is highly valuable in studying the role of TBK1 in various cellular signaling pathways, particularly those related to innate immunity and inflammation. Researchers employ HS-1371 to investigate its effects on the regulation of interferon production and its potential implications in autoimmune diseases and cancer. Additionally, it is used to explore the crosstalk between TBK1 and other signaling molecules, aiding in the development of targeted therapies for diseases where TBK1 activity is dysregulated. Its application extends to in vitro and in vivo studies, providing insights into the molecular mechanisms underlying immune responses and cellular stress pathways.