GCN2-IN-1 is primarily utilized in biomedical research to study the role of GCN2 (General Control Nonderepressible 2) kinase in cellular stress responses. It is widely used as a selective inhibitor to investigate the pathways involved in amino acid deprivation and the integrated stress response (ISR). Researchers employ it to understand its potential in modulating immune responses and tumor growth, particularly in cancer biology. Additionally, it serves as a tool compound to explore therapeutic strategies for diseases where GCN2 activity is implicated, such as certain cancers and metabolic disorders. Its application extends to preclinical studies aimed at evaluating its efficacy in enhancing the effectiveness of immunotherapies and chemotherapy.