DLK-IN-1 is primarily used in biological and medical research as a selective inhibitor of dual leucine zipper kinase (DLK). It plays a significant role in studying neurodegenerative diseases, particularly in understanding the mechanisms of neuronal apoptosis and axon degeneration. Researchers utilize DLK-IN-1 to investigate its potential therapeutic effects in conditions such as amyotrophic lateral sclerosis (ALS), glaucoma, and other disorders involving neuronal damage. Its application extends to preclinical studies where it helps in evaluating the efficacy of DLK inhibition in protecting neurons and promoting nerve regeneration. Additionally, DLK-IN-1 is employed in cellular assays to explore signaling pathways associated with DLK and its impact on cellular stress responses.