CKI-7 free base is widely used in biochemical and cellular research as a selective inhibitor of casein kinase I (CKI). It is particularly valuable in studying the role of CKI in various cellular processes, such as signal transduction, circadian rhythms, and cell cycle regulation. Researchers utilize this compound to investigate the phosphorylation mechanisms mediated by CKI and their impact on protein function. Additionally, CKI-7 free base is employed in studies exploring its potential therapeutic applications, including its effects on cancer cell proliferation and neurodegenerative diseases. Its ability to modulate CKI activity makes it a crucial tool for understanding the complex regulatory networks within cells.