CHIR-124 is primarily used in biochemical research as a potent and selective inhibitor of CHK1 (Checkpoint kinase 1). It is employed in studies focused on understanding the role of CHK1 in the DNA damage response and cell cycle regulation. Researchers utilize CHIR-124 to investigate how inhibition of CHK1 affects cancer cells, particularly in sensitizing them to DNA-damaging agents or radiation therapy. This makes it a valuable tool in exploring potential therapeutic strategies for cancer treatment. Additionally, CHIR-124 is used in mechanistic studies to dissect signaling pathways involving CHK1 and its interactions with other cellular components.