KU-60019 is primarily utilized in research settings as a potent and selective inhibitor of ATM kinase, a key protein involved in the DNA damage response. It is widely used in studies aimed at understanding the role of ATM in cellular processes, such as DNA repair, cell cycle regulation, and apoptosis. Researchers employ KU-60019 to investigate its potential in sensitizing cancer cells to radiation therapy or chemotherapy, as inhibiting ATM can disrupt the repair of DNA damage in tumor cells, making them more susceptible to treatment. Additionally, it is used in preclinical studies to explore its effects on neurodegenerative diseases, where ATM signaling pathways may play a role. Its application provides valuable insights into targeted cancer therapies and the mechanisms underlying cellular responses to DNA damage.