4-Nitroisoxazole is primarily utilized in the field of organic synthesis as a versatile intermediate. It plays a crucial role in the development of various pharmaceuticals, particularly in the synthesis of compounds with potential antibacterial and antifungal properties. Its nitro group makes it a valuable building block for constructing more complex molecules, often serving as a precursor in the creation of heterocyclic compounds. Additionally, it is employed in research settings to study chemical reactions and mechanisms involving nitro-substituted heterocycles, aiding in the discovery of new therapeutic agents.