Banoxantrone D12 is primarily used in research and development as a labeled compound for tracking and studying the pharmacokinetics and metabolism of banoxantrone, its parent compound. It serves as a stable isotope-labeled internal standard in mass spectrometry, enabling precise quantification and analysis of banoxantrone in biological samples. This application is crucial in preclinical and clinical studies to understand drug distribution, absorption, and elimination processes. Additionally, it aids in validating analytical methods and ensuring accuracy in drug development pipelines. Its use is particularly significant in oncology research, where banoxantrone is investigated for its potential therapeutic effects in cancer treatment.