Artesunate-d4, a deuterated form of artesunate, is primarily used in research and development as an internal standard in analytical chemistry. It plays a critical role in the quantification and analysis of artesunate and its metabolites in biological samples using techniques such as liquid chromatography-mass spectrometry (LC-MS). This ensures accurate and reliable measurements in pharmacokinetic studies, aiding in the understanding of drug metabolism, distribution, and excretion. Additionally, it is employed in the development and validation of analytical methods for quality control in pharmaceutical formulations, ensuring the efficacy and safety of artesunate-based therapies. Its use is particularly valuable in studies focusing on antimalarial drug development and resistance monitoring.