Used as an internal standard in analytical chemistry, particularly in mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy, for accurate quantification of nitroaromatic compounds. It aids in the study of environmental samples, detecting trace levels of nitrobenzene derivatives in water, soil, and air. Also employed in research to understand metabolic pathways and degradation mechanisms of nitroaromatic pollutants. Its deuterated form ensures minimal interference in isotopic labeling experiments, providing precise and reliable results.