Ambrisentan-d10 is primarily used in research and development settings as an internal standard in analytical chemistry. It is specifically employed in mass spectrometry and chromatography techniques to ensure accurate quantification and identification of ambrisentan in biological samples. This deuterated compound helps in distinguishing between the endogenous substance and the internal standard, improving the precision and reliability of pharmacokinetic studies. Its application is crucial in drug development and clinical trials, particularly for understanding the metabolism and distribution of ambrisentan in the body. Additionally, it aids in the validation of analytical methods, ensuring compliance with regulatory standards in pharmaceutical research.