(Rac)-Tenofovir-d6 is primarily used in research and development as a labeled internal standard in analytical studies. Its deuterated form allows for precise quantification and tracking of Tenofovir and its metabolites in biological samples using techniques like mass spectrometry. This is particularly valuable in pharmacokinetic studies, where understanding the absorption, distribution, metabolism, and excretion of Tenofovir is crucial. Additionally, it aids in the development and validation of sensitive and accurate assays for monitoring drug levels in patients, ensuring effective dosing and minimizing toxicity. Its application is also extended to forensic and environmental analysis, where detecting and quantifying trace amounts of the compound is essential.