Widely used as a stable isotope-labeled tracer in metabolic studies to track glucose metabolism pathways in biological systems. It is particularly valuable in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry for analyzing glucose utilization, glycolysis, and gluconeogenesis in cells, tissues, or organisms. In medical research, it helps investigate metabolic disorders such as diabetes and cancer by providing insights into glucose uptake and energy production. Additionally, it is employed in pharmaceutical development to study drug effects on glucose metabolism and in nutritional science to understand dietary glucose processing. Its isotopic labeling ensures accurate detection and quantification in complex biological matrices.