Used primarily in research and development, particularly in the field of analytical chemistry, it serves as an internal standard in mass spectrometry for the quantification of acridine and its derivatives. This compound is valuable in studies involving the metabolism and pharmacokinetics of acridine-based drugs, ensuring accurate and reliable measurements. Additionally, it aids in environmental monitoring by detecting trace amounts of acridine in various samples, contributing to the assessment of pollution levels and the effectiveness of remediation efforts. Its deuterated form enhances precision in these analytical applications due to its stable isotopic labeling.