Used in biomedical research to study cholesterol metabolism and its role in various diseases, particularly those related to the liver and cardiovascular system. It serves as a stable isotope-labeled internal standard in mass spectrometry, enabling accurate quantification of 7α-hydroxycholesterol in biological samples. This compound aids in understanding bile acid biosynthesis pathways and investigating disorders like cholestasis and atherosclerosis. Additionally, it is applied in pharmacological studies to evaluate the effects of drugs targeting cholesterol metabolism and related enzymes.