13C C16 Sphingomyelin (d18:1/16:0) is widely used in biomedical research as a labeled lipid tracer to study sphingolipid metabolism and membrane dynamics. Its stable isotope labeling allows for precise tracking and quantification in lipidomics studies, enabling researchers to investigate lipid pathways, cellular signaling, and membrane organization. It is particularly valuable in metabolic flux analysis to understand how sphingomyelins are synthesized, degraded, and transported within cells. Additionally, it serves as a critical tool in studying lipid-related diseases, such as cancer, neurodegenerative disorders, and metabolic syndromes, by providing insights into altered lipid metabolism. Its application extends to drug development, where it helps assess the efficacy of therapeutic agents targeting sphingolipid pathways.