Used as a stable isotope-labeled compound in metabolic studies and pharmaceutical research to trace biochemical pathways. Its primary application lies in nuclear magnetic resonance (NMR) spectroscopy, where the ¹³C label enhances signal detection for structural analysis. Also employed in mass spectrometry as an isotopically enriched internal standard for quantitative analysis of acetonitrile-containing samples. Widely utilized in environmental fate studies to monitor degradation processes without interference from natural abundance carbon signals.