Used primarily as a stable isotope-labeled tracer in research and analytical chemistry, this compound enables precise tracking of acrylamide metabolism and distribution in biological systems. It is essential in mass spectrometry studies for quantifying acrylamide and its metabolites in complex matrices such as blood, urine, and food samples, improving accuracy by serving as an internal standard. Its labeled structure allows differentiation from endogenous acrylamide, supporting toxicological and pharmacokinetic investigations. It is also employed in protein labeling and proteomics when incorporated into polyacrylamide gels, aiding in the identification and quantification of proteins with minimal interference in gel electrophoresis workflows.