Used as an internal standard in analytical chemistry for the accurate quantification of acesulfame potassium in food, beverages, and environmental samples. Its deuterated structure (with 4 deuterium atoms) allows for clear differentiation from non-labeled compounds in mass spectrometry, such as LC-MS/MS, improving measurement precision. Commonly applied in pharmacokinetic studies and metabolic research to track the presence and stability of artificial sweeteners in biological (e.g., plasma, urine, tissues) and environmental matrices. Also utilized in quality control during the production of sugar-free and low-calorie products to ensure consistent sweetener levels.