Used as a stable isotope-labeled analog of vitamin D3 in research and analytical studies. It serves as an internal standard in mass spectrometry for accurate quantification of vitamin D3 and its metabolites in biological samples. This compound is particularly valuable in clinical studies to assess vitamin D status, metabolism, and its role in health and disease. It aids in developing and validating methods for detecting vitamin D levels in blood, urine, and other tissues. Additionally, it supports investigations into the bioavailability and pharmacokinetics of vitamin D3 supplements.