Used as a stable isotope-labeled internal standard in mass spectrometry and gas chromatography for the detection and quantification of methyl acetate and related compounds in biological, environmental, or industrial samples. Its deuterated form (with three deuterium atoms in the methyl group) allows for accurate differentiation from non-labeled compounds during analytical testing. Commonly applied in metabolic studies, toxicology screenings, food safety analysis, and environmental monitoring where precise tracking of ester-derived compounds is required. Also employed in synthetic chemistry and kinetic studies to trace reaction pathways involving acetate esters without altering chemical behavior due to isotopic labeling.