Used primarily in organic synthesis and pharmaceutical research, this compound serves as a key reagent for introducing isothiocyanate functionality in the development of bioactive molecules. Its protected phenyl ring allows for selective reactions in multi-step syntheses, particularly in the preparation of thiourea derivatives through reaction with amines. These thioureas are often explored for their biological activity, including enzyme inhibition and receptor binding. The tert-butoxycarbonyl (Boc) group provides stability and compatibility with various reaction conditions, making the compound valuable in solid-phase synthesis and combinatorial chemistry. It is also employed in the labeling and modification of peptides and proteins for biochemical assays.