6,9-Difluorobenzo[g]isoquinoline-5,10-dione is primarily utilized in the field of organic synthesis and pharmaceutical research. It serves as a key intermediate in the development of various biologically active compounds, particularly those targeting cancer therapies. Its structure is often exploited in the design of molecules that can interact with DNA or specific enzymes, making it valuable in the creation of chemotherapeutic agents. Additionally, it is explored in the synthesis of fluorescent dyes and probes due to its unique photophysical properties, which are useful in bioimaging and diagnostic applications. Researchers also investigate its potential in material science, particularly in the development of organic semiconductors and optoelectronic devices.