Used in the biosynthesis of benzylisoquinoline alkaloids, N-Methylcoclaurine serves as a key intermediate in the production of pharmacologically active compounds such as morphine, codeine, and berberine. It plays a critical role in plant-based metabolic pathways, particularly in opium poppy and related species, enabling the formation of complex alkaloids with analgesic, antitussive, and antimicrobial properties. Its enzymatic conversion by cytochrome P450s and methyltransferases is studied for metabolic engineering and sustainable production of medicinal alkaloids in synthetic biology systems.