Alamethicin is widely used in research and biotechnology due to its ability to form ion channels in lipid bilayers, making it a valuable tool for studying membrane permeability and ion transport. It is often employed in electrophysiology experiments to investigate the mechanisms of channel formation and function. Additionally, alamethicin is used in the development of biosensors and drug delivery systems, where its pore-forming properties can be harnessed to control the release of molecules. Its antimicrobial activity also makes it a subject of interest in the study of peptide-based antibiotics and potential therapeutic applications.