Used as a mobile phase additive in reversed-phase high-performance liquid chromatography (HPLC) for the separation of peptides and other basic compounds. The combination helps control ionization and improves peak shape by acting as an ion-pairing agent. Commonly applied in peptide purification and analysis due to its effectiveness in enhancing resolution and reproducibility. Also utilized in organic synthesis as a reagent mixture for deprotection and coupling reactions, especially in solid-phase peptide synthesis. The 2M:2M ratio ensures consistent buffering capacity and pH control during processes.