DEAE-52 is widely used in biochemistry and molecular biology for the purification of proteins, nucleic acids, and other biomolecules. Its primary application is in ion exchange chromatography, where it serves as an anion exchange resin. The diethylaminoethyl (DEAE) groups on the cellulose matrix attract negatively charged molecules, allowing for the separation and purification of target compounds based on their charge properties. It is particularly effective for isolating enzymes, antibodies, and plasmid DNA from complex mixtures. DEAE-52 is also employed in the fractionation of serum proteins and the removal of contaminants during downstream processing. Its high capacity and compatibility with a wide range of buffers make it a versatile tool in both research and industrial settings. Additionally, it is often used in the initial steps of protein purification workflows to concentrate and partially purify samples before further refinement.