SP-Sepharose Fast Flow is widely used in the purification of proteins, particularly in the biotechnology and pharmaceutical industries. Its strong cation exchange properties make it ideal for separating and purifying positively charged biomolecules from complex mixtures. It is commonly employed in the initial capture and intermediate purification steps of monoclonal antibodies, enzymes, and other therapeutic proteins. The fast flow characteristics enable high throughput processing, making it suitable for large-scale production. Additionally, it is used in laboratory research for protein analysis, fractionation, and sample preparation due to its high binding capacity and resolution. Its robustness and consistency make it a reliable choice for both industrial and academic applications.