Benzamil is primarily used in research settings to study ion channels, particularly sodium channels, due to its ability to block these channels effectively. It is often employed in physiological studies to understand the role of sodium channels in various cellular processes, including fluid transport and sensory functions. Additionally, Benzamil has been investigated for its potential therapeutic applications, particularly in conditions like cystic fibrosis, where it may help regulate sodium and fluid balance in the lungs. Its use in experimental models also extends to exploring its effects on hypertension and kidney function, given its influence on sodium reabsorption.