GSK101 is primarily utilized in scientific research to study the TRPV4 ion channel, which plays a critical role in various physiological processes. It acts as a potent and selective agonist for TRPV4, enabling researchers to investigate the channel's involvement in mechanosensation, osmoregulation, and pain signaling. This compound is particularly valuable in understanding the mechanisms underlying conditions like edema, inflammation, and neurodegenerative diseases. Additionally, GSK101 is used in pharmacological studies to explore potential therapeutic targets for diseases linked to TRPV4 dysfunction, such as osteoarthritis and pulmonary disorders. Its application in experimental models helps uncover insights into cellular responses to mechanical and osmotic stress, contributing to advancements in drug development and biomedical research.