QX-314 bromide is primarily used in neuroscience research as a selective sodium channel blocker. It is particularly valuable in studying pain pathways and neuronal excitability. When applied intracellularly, it inhibits sodium channels, effectively silencing specific neurons without affecting surrounding cells. This allows researchers to investigate the role of specific neuronal populations in pain sensation and other neural processes. Its use has also been explored in experiments involving local anesthesia, as it can provide targeted pain relief when combined with agents that facilitate its entry into cells. Additionally, QX-314 bromide is utilized in electrophysiological studies to dissect the mechanisms of action potential generation and propagation in neurons.