ML277 is primarily used in research settings to study potassium channels, particularly the KCNQ family. It acts as a potent and selective activator of KCNQ2/3 channels, which are crucial for regulating neuronal excitability. This makes it a valuable tool for investigating conditions related to neuronal hyperexcitability, such as epilepsy and neuropathic pain. Additionally, ML277 is utilized in drug discovery efforts to develop targeted therapies for neurological disorders by modulating potassium channel activity. Its specificity allows researchers to better understand the role of KCNQ2/3 channels in cellular and physiological processes.