Madrasin is a potent small-molecule inhibitor that specifically targets the abscission step of cytokinesis in mammalian cells. It disrupts the protein-protein interaction between CEP55 and ALIX, preventing ESCRT-III complex recruitment required for midbody resolution. This results in midbody accumulation and binucleated cell formation. Madrasin is extensively used in cell biology research to study cytokinesis machinery, ESCRT pathway functions, and their roles in cancer biology, viral budding, and tissue homeostasis. It serves as a key tool compound for dissecting late-stage cell division mechanisms.