Precocene II is primarily used in insect research due to its ability to disrupt insect development and reproduction. It acts as an anti-juvenile hormone, accelerating the transition from juvenile to adult stages in insects, which can lead to premature metamorphosis and reduced fertility. This property makes it valuable in studying insect endocrinology and development.

It has been investigated for its potential as a natural insect growth regulator in pest control, especially against species like mosquitoes and agricultural pests. By interfering with hormonal balance, Precocene II can prevent larvae from developing properly, reducing pest populations without the use of conventional insecticides.

Additionally, Precocene II exhibits anti-cancer and anti-inflammatory properties in preliminary biological studies. It has shown cytotoxic effects against certain tumor cell lines, prompting interest in its use as a lead compound in pharmaceutical research. However, these applications remain largely experimental.

Due to its role in blocking juvenile hormone activity, it is also used as a tool in entomological studies to understand aging, reproduction, and metamorphosis in insects.