Used in research for studying ecdysteroid signaling pathways in insects and plants. Acts as a stable analog to natural ecdysteroids, such as makisterone A, making it valuable for investigating molting, development, and gene regulation processes. Its 20,22-monoacetonide modification enhances metabolic stability, allowing longer-lasting effects in experimental settings. Commonly applied in biochemical assays, insect physiology studies, and development of insect growth regulators for pest control.