20-Hydroxyecdysone 3-acetate is a semi-synthetic derivative of 20-hydroxyecdysone, a key insect molting hormone (ecdysteroid). Primarily used in entomological and biological research to mimic or modulate ecdysone signaling, studying processes like molting, development, and hormone pathways in insects and cell models. It is employed to stimulate or inhibit physiological changes across insect life stages. Additionally, ecdysteroids including this derivative are investigated for bioactive effects in vertebrates, such as promoting protein synthesis, muscle regeneration, and anabolic activity, though still in experimental stages. In cosmetic and dermatological applications, related ecdysteroids support skin cell regeneration, collagen synthesis, improved elasticity, reduction of fine lines, wound healing, and anti-inflammatory effects, often in anti-aging formulations.