Used primarily in research settings, this compound serves as a protected derivative of ecdysterone with 2,3:20,22-diacetonide groups, facilitating studies on ecdysteroid signaling pathways in insects and other arthropods. Its acetonide protection enhances stability and solubility, making it suitable for synthetic and biochemical applications where precise manipulation of ecdysteroid activity is required. It is commonly used in cell culture systems to stimulate gene expression responsive to ecdysteroids and in developing inducible protein expression systems in molecular biology research. Additionally, it is explored in the development of bioactive molecules for potential use in agriculture, such as growth regulators or non-toxic pest control agents. Due to the anabolic properties associated with ecdysteroids, this derivative may support investigations into muscle growth and metabolic regulation, though such uses remain experimental and are not approved for human therapeutics.