5-Lipoxygenase-In-1 is primarily used in research settings to study the 5-lipoxygenase pathway, which plays a significant role in the biosynthesis of leukotrienes. Leukotrienes are inflammatory mediators involved in various inflammatory and allergic conditions, such as asthma, rheumatoid arthritis, and inflammatory bowel disease. By inhibiting 5-lipoxygenase, this compound helps researchers understand the mechanisms underlying these conditions and explore potential therapeutic strategies. Additionally, it is utilized in pharmacological studies to evaluate the efficacy of new anti-inflammatory drugs targeting the 5-lipoxygenase pathway. Its application extends to cellular and biochemical assays to investigate the molecular interactions and signaling pathways associated with inflammation and immune responses.