TLR7/8-IN-1 is primarily used as a potent and selective inhibitor of Toll-like receptors 7 and 8 (TLR7/8), which play key roles in the innate immune response. It is widely applied in immunological research to modulate TLR7/8-mediated signaling pathways, helping to study the involvement of these receptors in inflammatory and autoimmune diseases. By suppressing the activation of NF-κB and the production of pro-inflammatory cytokines such as TNF-α, IL-6, and IFN-α, this compound is useful in investigating conditions like systemic lupus erythematosus, rheumatoid arthritis, and other TLR-driven pathologies. It also serves as a tool compound in drug development for evaluating the therapeutic potential of targeting TLR7/8 in both inflammatory disorders and certain viral infections where TLR overactivation contributes to disease progression.