Compound 48/80 hydrochloride is widely used in research and experimental studies as a potent mast cell degranulator. It is particularly valuable in studying allergic reactions, inflammation, and immune responses, as it mimics the effects of allergens by triggering the release of histamine and other mediators from mast cells. This property makes it a useful tool in pharmacological and immunological research to understand mast cell behavior and develop treatments for conditions like asthma, allergies, and autoimmune disorders. Additionally, it is employed in preclinical studies to evaluate the efficacy of anti-allergic and anti-inflammatory drugs. Its ability to induce controlled mast cell activation provides insights into the mechanisms underlying hypersensitivity and immune system regulation.