4,5-Dichloroisatin is primarily utilized in organic synthesis as a versatile intermediate for the preparation of various heterocyclic compounds. It serves as a key building block in the development of pharmaceuticals, particularly in the synthesis of compounds with potential biological activities, such as antiviral, antibacterial, and anticancer agents. Additionally, it is employed in the creation of dyes and pigments due to its ability to form stable and complex structures. Its reactivity with nucleophiles and electrophiles makes it valuable in constructing diverse chemical frameworks for research and industrial applications.