6-Methylisatin is widely used as a key intermediate in the synthesis of various biologically active compounds, particularly in pharmaceutical research. It serves as a building block for indole-based derivatives, which are important in the development of anticancer, antimicrobial, and antiviral agents. Its structure allows for easy functionalization, making it valuable in creating compound libraries for drug discovery. Additionally, 6-methylisatin has been investigated for its role in synthesizing kinase inhibitors and other enzyme modulators due to its ability to interact with biological targets through hydrogen bonding and aromatic interactions.