Murexide is widely used as a complexometric indicator in analytical chemistry, particularly in the titration of calcium and other metal ions. It changes color in the presence of specific metal ions, making it valuable for determining endpoints in complexometric titrations, especially with EDTA. In biological research, it serves as a staining agent for identifying calcium deposits in tissues. Additionally, murexide is employed in the dye industry for coloring textiles and as a pH indicator due to its distinct color changes in different pH ranges. Its versatility in detecting and visualizing metal ions makes it a crucial tool in both laboratory and industrial settings.